Part:BBa_K4316028

5(4GlySer) linker + TEV cleavage site B4

The 5(4GlySer) linker and TEV cleavage site were designed to be used with the Chlamydomonas Reinhardtii MoClo kit [1] and are in position B4. Their 3’ tag is ggTTCG as to maintain correct reading frame.

It serves as a TEV-specific cleavage site between nuclease A and transmembranary domain . The 5(4GlySer) linker, inspired by IGEM Munich 2013's team, was wrongly annotated. We corrected the annotation, created a basic part for it, and deleted the excedentary sequence.

Sources:

[1] Crozet, Pierre & Navarro, Francisco & Willmund, Felix & Mehrshahi, Payam & Bakowski, Kamil & Lauersen, Kyle & Pérez-Pérez, María & Auroy, Pascaline & Gorchs Rovira, Aleix & Sauret-Gueto, Susana & Niemeyer, Justus & Spaniol, Benjamin & Theis, Jasmine & Trösch, Raphael & Westrich, Lisa & Vavitsas, Konstantinos & Baier, Thomas & Hübner, Wolfgang & de Carpentier, Félix & Lemaire, Stéphane. (2018). Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology. 7. 10.1021/acssynbio.8b00251.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]